MBFA9对蛋白核小球藻生长的影响研究

以蛋白核小球藻(Chlorella pyrenoidosa)为实验生物,以生物量、叶绿素含量、可溶性蛋白含量及抗氧化酶活力的变化为指标,研究了不同浓度的高效微生物絮凝剂MBFA9对蛋白核小球藻的毒性效应,结果表明:低浓度短时间内MBFA9可以抑制蛋白核小球藻的生长,随着时间的延长,抑制作用减弱并最终消失;高浓度MBFA9最终可以轻微促进蛋白核小球藻生长,但SOD比活力和POD比活力稳定,表明未启动抗氧化酶系统和非酶抗性系统,对藻细胞不构成伤害。初步证明,絮凝剂MBFA9无急毒反应,是一种安全的、无毒的、对生态环境友好的微生物发酵制品。

This paper dealt with the studies on environmental toxicological characteristics of polysaccharide mierobial floeeulant by Chlorella pyrenoidosa with the indicators of the biomass, chlorophyll content （Chl-a, Chl-b）, soluble protein content and antioxidant enzyme activity （SOD, POD）.The results showed that： low concentrations of MBFA9 could inhibit the growth of Chlorella pyrenoidosa at the very start, but the inhibiting effects weaken with time and disappear eventually; high concentrations of MBFA9 could promote the growth of Chlorella pyrenoidosa slightly. Meanwhile, the activities of superoxide dismutase（SOD） and peroxidase（POD）keep stable. It showed that the antioxidase system and the nonenzyme-resistance system were not started, and the algal cells were not injured. It was primarily testified that MBFA9 was a safe, nontoxic, eeo-friendly microbial fermentation product.