摘要
采用3种提取植物组织蛋白的方法(TCA/丙酮沉淀法、E-TCA法、酚法)提取槟榔叶片总蛋白,在蛋白产量,一维和二维电泳图谱等方面对3种方法的提取效果进行比较。结果表明,TCA/丙酮沉淀法效果较好,是槟榔叶片总蛋白提取的可选方法,同时优化了双向电泳的条件和上样量。
Proteins from betel nut （A reca catechu Linn.）were extracted by using three methods [trichloroacetic acid （TCA）/acetone precipitation, trichloroacetic acid precipitation （E-TCA）, and phenol extraction], and their productivity and profiles were assessed by means of SDS-PAGE and two-dimensional electrophoresis. Of these 3 methods the TCA/acetone precipitation method was found most effective for the extraction and two-dimensional electrophoresis of proteins from A reca leaves. The conditions of two- dimensional electrophoresis and protein loading volume were optimized.
出处
《热带作物学报》
CSCD
2009年第6期746-750,共5页
Chinese Journal of Tropical Crops
基金
国家科技支撑计划课题"热带农林重要有害生物检测监测预警技术研究"(No.2007BAD48B01)资助
关键词
槟榔
蛋白提取
二维电泳
Areca catechu Linn.
protein extraction
two-dimensional electrophoresis.
