摘要
目的对添加海藻糖的血小板添加液替代70%血浆冷藏的血小板进行动物体内输注效果评价。方法采集兔心脏血,按常规方法制备浓缩血小板,将浓缩血小板分3组保存。血浆常温组:添加100%血浆,22℃保存;添加液低温组:添加70%PAS-ⅢM/30%血浆,10℃保存;冰冻保存组:添加100%血浆,-85℃保存。以新鲜血小板为对照,常温血浆保存组在d 3,添加液低温保存组在d 3、7、9,冰冻保存组在d 20复苏后分别检测血小板缺乏兔模型的血小板24 h回收率和生存率。结果除保存9 d的添加液低温组血小板存活率明显低于新鲜血小板存活率(P<0.05)外,血浆常温组、添加液低温组以及新鲜血小板的24 h回收率和存活率相互比较差异均无显著统计学意义(P>0.05)。冰冻保存组血小板24 h回收率和存活率均低于其他各组(P<0.05)。结论改良的PAS-ⅢM能够替代血浆在低温条件下用于血小板的保存,能维持血小板的体内功能。
Objective To evaluate the in vivo efficacy of modified platelet （PLT） additive solution （PAS-ⅢM ） with trehalose as a substitute for plasma for the storage of platelet concentrates at low temperature （ 10℃ ）. Methods Rabbit platelet concentrates were collected and stored in different media and temperature conditions, plasma （22℃）, 70% PAS-Ⅲ M/30% plasma （10℃） and plasma/trehalose （ -85℃ ）. Platelets in plasma （22℃） on storage day 3, in 70% PAS-Ⅲ M/30% plasma （ 10℃） on storage day 3, 7, 9, and in plasma/trehalose （ - 85℃ ） thawed on day 20 were transfused to rabbit thrombocytopenia model. The in vivo recoveries and survivals of the three preserved platelets and fresh platelets were measured and evaluated after transfusion into rabit model of thromboeytopenia. Results The survival of platelets stored in 70% PAS-ⅢM/30% plasma（ 10℃） on day 9 was lower than that of fresh platelets （P 〈 0.05 ）. The recoveries and survivals of platelets stored in plasma （22~C）, in 70% PAS-Ⅲ M/30% plasma （10℃） on day 3 and day 7 were not different from fresh platelets （22℃）（P 〉 0.05）. Recovery and survival of frozen platelets were significant lower than those of the other groups （P 〈 0.05）. Conclusion PAS-Ⅲ M with trehalose can protect the chilled rabbit platelets, prolong the circulation of refrigerated rabbit platelets, and could be a good substitute for plasma for platelet storage.
出处
《中国输血杂志》
CAS
CSCD
北大核心
2009年第8期646-649,共4页
Chinese Journal of Blood Transfusion
基金
第三军医大学西南医院临床创新课题(2008002)
