摘要
A method of determination by UV spectrophotometry and identification by thin - layer chromatography of tetracaine hydrochloride eye drop was established. The maximum absorbance wavel ength at 310 ± 1nm was selected for determination wavelength, ethyl hydroxybenzoate has no interferrence. The result showed that the absorbance coeffecient (E1cm1%) of tetracaine hydrochloride at 310 ± 1nm was 760,RSD = 0.5% （n = 20）, the mean recovery for sample was 99.5%, RSD = 0.5% （n = 7）. This method is simple, rapid, accurate and reproducible.
A method of determination by UV spectrophotometry and identification by thin - layer chromatography of tetracaine hydrochloride eye drop was established. The maximum absorbance wavel ength at 310 ± 1nm was selected for determination wavelength, ethyl hydroxybenzoate has no interferrence. The result showed that the absorbance coeffecient (E_(1cm)^(1%)) of tetracaine hydrochloride at 310 ± 1nm was 760,RSD = 0.5% (n = 20), the mean recovery for sample was 99.5%, RSD = 0.5% (n = 7). This method is simple, rapid, accurate and reproducible.
出处
《药学实践杂志》
CAS
1998年第4期230-232,共3页
Journal of Pharmaceutical Practice
关键词
滴眼液
盐酸丁卡因
薄层色谱法
紫外光谱法
tetracaine hydrochloride, UV spectrophotometry, thin - layer chromatography
