母婴分离对大鼠成年后海马星形胶质细胞的影响

Maternal deprivation modulates hippocampal astrocytes of rats in adulthood

【目的】研究生后早期长期反复母婴分离对子代大鼠成年后海马星形胶质胶质细胞的影响。【方法】新生大鼠分为母婴分离组和对照组,每组分别有雄性和雌性大鼠各10只。母婴分离组大鼠于生后第2 d至生后第14 d每日与母鼠分离3 h,对照组大鼠不受干扰。各组大鼠于生后第60 d处死,采用免疫组化方法分析各组大鼠背侧和腹侧海马星形胶质细胞标记物(GFAP和S100β)的表达。【结果】双因素方差分析显示:与对照组相比,母婴分离组雄性大鼠成年后腹侧海马GFAP和S100β染色阳性细胞数显著减少(P<0.05);背侧海马星形胶质细胞中GFAP和S100β平均光密度明显下降(P<0.001)。【结论】母婴分离可使子代雄性大鼠成年后腹侧海马星形胶质细胞数量减少,背侧海马星形胶质细胞中GFAP和S100β含量下降。

[Objective] To investigate possible influences of early-life maternal deprivation on hippocampal astroeytes in rats in adulthood. [ Methods] Newborn rats were divided into maternal deprivation group and control group, each group containing 10 male and 10 female rats. Rats in maternal deprivation group were separated from their dams for 3 hours each day from postnatal day 2 to postnatal day 14. Controls were not disturbed. Two astrocytie markers, GFAP and S100β, were immunostained and compared in dorsal and ventral hippoeampi separately. [Results] Two-way ANOVA analysis revealed that the number of GFAP and S100β positive cells in ventral hippocampi in male rats were significantly smaller in maternal deprivation group than those in control（P〈0.05） and the average optical density for GFAP and S100β in dorsal hippocampi in male rats were markedly lower in maternal deprived rats than those in eontrols（P〈0. 001 ）. [Conclusion] Early life maternal deprivation could reduce the number of astroeytes in ventral hippoeampi and deduce the content of GFAP and S100β in astrocytes in dorsal hippocampi in adult male rats.