脑星形细胞瘤增殖活性的研究

The study of proliferative activities of astrocytomas

目的研究脑星形细胞瘤增殖活性，探讨ＰＣＮＡ－ＬＩ、ＡｇＮＯＲ计数与肿瘤恶性程度和病理学分级的关系。方法应用ＰＣＮＡ免疫组化染色和ＡｇＮＯＲ染色测定５０例脑星形细胞瘤的ＰＣＮＡ－ＬＩ和ＡｇＮＯＲ计数。结果Ⅰ～Ⅳ级星形细胞瘤的ＰＣＮＡ－ＬＩ分别为２８．６±１３．７５％，４５．７５±２２．１４％，６４．３８±１９．１７％和８３．７０±６．９９％，各级之间存在显著差别；ＡｇＮＯＲ计数分别为１４１．５２±２０．６９，１５８．４４±２８．００，１８７．３８±２７．７１，２２５．２±３６．５３，Ⅰ～Ⅱ级与Ⅲ级、Ⅳ级及Ⅲ级与Ⅳ级相比均有显著差别。结论ＰＣＮＡ－ＬＩ是评估星形细胞瘤增殖活性的良好指标。ＡｇＮＯＲ染色简便、快速，ＡｇＮＯＲ计数大于２００。

オbjective To elucidate the correlation of PCNALI and AgNOR counts with the malignancy and pathological grade of astrocytomas by investigating the proliferative activities of astrocytomas.Methods PCNA immunostaining and AgNOR staining were employed to study the fresh specimens from 50 cases of astrocytomas.Results PCNALI of astrocytomas grade Ⅰ～Ⅳ were28.64±13.75%,45.75±22.14%,64.38±19.17%,and 83.70±6.99% respectively, showing statistical difference between each grade in PCNALI.Meanwhile,AgNOR counts of grade ⅠⅣ were 141.52±20.69,158.44±28,187.38±27.71,and 225.2±36.53.There was significant difference in the AgNOR counts among grade ⅠⅡ and grade Ⅲ、grade Ⅳ.There also existed significant difference in the AgNOR counts between gradeⅢ and grade Ⅳ.Conclusion PCNALI was a good index in evaluating the proliferative activities of astrocytomas,and AgNOR staining was an economic and quick method.AgNOR counts larger than 200 showed malignant evidence of astrocytomas.