用聚合酶链反应技术检测泌尿系疾病患者尿中疱疹病毒DNA

Detection of Herpesvirus DNA in Urine Pellet of Patients with Urinary System Disorders

为探讨泌尿系疾病与疱疹病毒感染的关系，我们用ＰＣＲ及限制性内切酶酶切技术检测了尿中疱疹病毒的ＤＮＡ。９９例泌尿系疾病患者中，５８例检出ＣＭＶ；对照组５２例中，８例检出ＣＭＶ，两组检出率间有显著性差异，但不同病种ＣＭＶ检出率之间差异无显著性。患者组和对照组尿中均未检出ＥＢ病毒（ＥＢＶ），Ⅰ型单纯疱疹病毒（ＨＳＶⅠ）和Ⅱ型单纯疱疹病毒（ＨＳＶⅡ）。３５例患者同时作了血清抗“Ｏ”检测，１６例急性肾炎，８例慢性肾炎和９例肾病综合征中，抗“Ｏ”阳性率分别为５００％、１２５０％和１１１１％，相应的ＣＭＶ阳性率则分别为６２５０％、６２５０％和５５５６％。

In order to observe the relationship of urinary system disorder with herpesvirus infection, DNA of herpesvirus was detected in 99 cases with urinary system disorders by methods of PCR and RFLP.DNA of herpesvirus was positive in 58 out of 99 cases and 8 out of 52 control group.There were significent difference in the two groups. DNA of EBV,HSV Ⅰ and HSV Ⅱ were negative in these two groups.Anti Streptococcus “O” hemolysin was tested in the 35 patients inclued 16 acute glomerulonephritis patients,8 chronic glomerulonephritis patients and 9 nephrotic syndrome patients,positive rate were 50%,12.5%and 11.1% in the samples, and their CMV positive rate were 62.5%,62.5% and 55 56%,respectively.The results showed that glomerulonephritis was associated with streptococcus. But CMV may be involved to many kind of urinary system disorders. However we have not known if CMV is primary infection or acquired infection in urinary system disorder.It is necessary to further study.