高效液相色谱法测定椰油酰胺丙基甜菜碱中单氯乙酸钠

Determination of sodium monochloroacetate in cocofatty acylamido propyl betaines by HPLC

采用高效液相色谱法（HPLC）对椰油酰胺丙基甜菜碱中单氯乙酸钠的残留量进行分析测定。首先用1－丁醇对样品进行预处理，然后用HPLC外标法测定。色谱条件：有机酸分析柱，流动相为0．005mol／L稀硫酸，流速0．6ml/min，紫外检测波长200nm，柱温35℃。结果表明，在此条件下，线性回归方程为Y=1409x＋733，线性范围2μg·g^-1～200μg·g^-1，r=0．9999，相对标准偏差为1．68％，测试回收率为96．0％～106．0％。

A HPLC method for the determination of sodium monochloroacetate in cocofatty acylamido propyl betaines was established. Samples of cocofatty aeylamido propyl betaines were extracted using 1 - butanol as solvent. The extract was analyzed using a HPLC external standard method with UV detection wavelength at 200 nm. The mobile phase is 0. 005 mol/L sulfuric acid solution,at a flow rate of 0. 6 mL/min ,column temperature is 35℃. Result showed that as the concentration of sodium chloroacetate is in the range of 2 μg ·g^-1 - 200 μg·g^-1 ,r =0.999 9,the regression equation can be expressed as y = 1 409x ＋ 733 and the standard deviation is 1.68%. Recovery achieves 96. 0% - 106. 0% .