感染Coxsackie B_(3m)病毒小鼠心肌NOS_Ⅱ蛋白表达的研究

The expression of protein NOSⅡ in myocardium of mice with infected tropism mice myocardium Coxsackie B_3 virus

给ＢＡＬＢ／Ｃ小鼠腹腔接种嗜鼠心肌ＣｏｘｓａｃｋｉｅＢ３病毒（ＣＶＢ３ｍ），感染后３、５、７、１２、１５、２０、３０天分批处死小鼠。应用免疫组化法检测发现，感染后５天，小鼠心肌可见ＮＯＳⅡ蛋白的阳性表达，７～１５天时表达最明显，２０天后逐渐减少。表达ＮＯＳⅡ蛋白的细胞主要位于炎性病灶的周围。

The tropism mice myocardium Coxsackie B_3 virus(CVB3m) was inoculated into abdominal cavity of BALB/C mice,then the mice were killed in 3,5,7,9,12,15,20,and 30 days after infection.By immunohistochemical method,we found the expression of protein NOSⅡ in the myocardium of mice after 5 days.There were cells of experssion of protein NOSⅡ around inflammatory focus.The result suggested that NO participate in occurrence and development of myocardial in jure of mice.