摘要
用ＳＤＳ－ＰＡＧＥ结合电渗洗脱的方法分离、纯化日本血吸虫重组３２ｋＤ抗原（ｒＳｊ３２）；用酶联免疫印迹技术（ＥＩＴＢ）分析纯化抗原的免疫活性。用纯化抗原、日本血吸虫成虫抗原（ＡＷＡ）和可溶性虫卵抗原（ＳＥＡ）分别检测慢性血吸虫病人、肺吸虫病人、肝吸虫病人、钩虫病人和健康人血清抗体。结果表明：纯化的ｒＳｊ３２分子量为３７ｋＤ，有较好的免疫活性；用其检测日本血吸虫病的敏感性和特异性与虫源性抗原（ＡＷＡ和ＳＥＡ）无显著性差异。
Separation and purification of rSj32 were carried out with SDSPAGE and electroelution technique. The purified rSj32, AWA and SEA were used to examine sera of patients with schistosomiasis, helthy individuals and patients with other parasitic diseases by ELISA. The results showed that the molecule weight of rSj32 was 37kD. The sensitivity and specificity of rSj32 as a coating antigen in ELISA were as good as those of AWA and SEA. It is suggested that rSj32 antigen is a potential antigen for immunodiagnosis of schistosomiasis japonica.
出处
《湖南医科大学学报》
CSCD
1998年第4期331-334,共4页
Bulletin of Hunan Medical University
基金
国家科委"九五"攻关项目
JRMC项目
关键词
日本血吸虫
日本血吸虫病
纯化
rSj32
Schistosoma japonicum
schistosomiasis
recombinant Sj32 antigen*
diagnostic use
