大鼠感染性脑损伤中神经细胞内游离钙的变化

Alterations of intracellular free calcium concentration of cerebralneuronal cells in infectious brain injury in rats

采用荧光法测定负载Ｆｕｒａ－２／ＡＭ大脑皮层突触体内游离钙［Ｃａ２＋］ｉ浓度在大鼠感染性脑损伤（感脑）中的变化及其意义。结果显示：百日咳菌液组（０．５ｈ，４ｈ，２４ｈ）的［Ｃａ２＋］ｉ，含水量（ＷＣ）及伊文思蓝（ＥＢ）含量均高于对应盐水对照组（Ｐ＜０．０５或＜０．０１）；菌液组［Ｃａ２＋］ｉ增高呈时效关系，以２４ｈ组含量最高（４ｈｖｓ０．５ｈ，Ｐ＜０．０５；２４ｈｖｓ４ｈ，Ｐ＜０．０１），并分别与ＷＣ及ＥＢ含量呈高度正相关（０．５ｈ，４ｈ，Ｐ＜０．０１）及无相关（２４ｈ，Ｐ＞０．０５）。结果表明。

Intracellular free calcium concentration, [Ca^(2+)]i, of cerebral synaptosomes loaded by Fura2/AM was measured by fluorescent method to clarify alterations of [Ca^(2+)]i in infectious brain injury (IBI) in rats. The results showed that [Ca^(2+)]i, water content (WC), and Evans blue content (EB) in groups injected by Bordetella pertussis suspension (BP) were increased compared to those in groups injected by normal saline (NS) at 0.5h, 4h, and 24h, respectively. Increased [Ca^(2+)]i in BP groups showed time-dependent relationship and the highest level in the 24h group. There was positive correlation (0.5h, 4h) and no correlation (24h) between [Ca^(2+)]i and WC or EB. It is suggested that [Ca^(2+)]i accumulation of cerebral neuronal cells plays an important role in the pathogenetic mechanism of the acute and delayed IBI induced by BP.