用PCR检测人淋巴组织中弓形虫

Polymerase chain reaction applied for detection ofToxoplasma gondii in lymph nodes

将淋巴结病理组织提取ＤＮＡ后，用Ｔｇ－ＤＮＡＰＣＲ药盒体外扩增弓形虫ＤＮＡ。结果显示，１２０例淋巴结病理标本中，７例可检出２１０ｂｐ的扩增产物，其中３例ＨＤ（３／３２），２例ＮＨＬ（２／４１），２例ＣＬ（２／４７）。取ＰＣＲ扩增产物进行Ｓｏｕｔｈｅｒｎ－ｂｌｏｔ杂交试验（ＰＣＲ－ＳＢＨ），发现除原有７例阳性外，ＣＬ患者中另１例阳性；总检出率为６．６７％（８／１２０）。ＨＤ，ＮＨＬ及ＣＬ的阳性率分别为９．３８％，４．８８％和６．３８％。

In order to investigate the morbidity of toxoplasmic lymphadenitis, polymerase chain reaction (PCR) was used to detect DNA of Toxoplasma gondii within lymph nodes in 3 groups of 120 patients with different diseases. After extracting the DNA of each sample, PCR was employed to amplify toxoplasma DNA. The results showed that the amplification product of 210bp was confirmed in 7 patients: 3 cases of Hodgkin's disease (HD), 2 cases of nonHodgkin's lymphoma (NHL) and 2 cases of chronic lymphadenitis (CL). Each PCR product was then subjected to Southern blot hybridization. Besides the 7 cases proved by PCR, 1 case of CL was found positive. The positive percentages of HD, NHL and CL were 9.38% (3/32), 4.88% (2/41), and 6.38% (3/47), respectively. The total positive rate was 6.67% (8/120).