摘要
采用新型Ca2+荧光指示剂Fura-2,测定冷冻伤性脑水肿时突触体内游离钙的离子浓度。
Intracellular free calcium [Ca^(2+)]i concentration has become a focus of attention for investigators in the field of brain edema. It is the purpose of the present study that the changes of the concentration of the free calcium within cortical neurons were investigated on the model of brain edema induced by freezing injury. [Ca^(2+)]i was determined in calcium fluorescent indicator Fura-2/AM loaded neuronal synaptosomes by using monowave length spectrofluorophotometer. The method for preparing synaptosomes, and the method and mechanism of concentration of cytosolic free calcium using fluorescentindicator Fura-2/AM were studied.
出处
《湖南医科大学学报》
CSCD
1998年第4期375-378,共4页
Bulletin of Hunan Medical University