摘要
介绍一种ＰＣＲ最优化方法，即正交设计直观分析法，旨在摸索ＰＣＲ的最佳实验条件。结果证明该法科学而简便。
Polymerase chain reaction (PCR) optimization is difficult but very important. It is hard to gain the optimal condition due to the confussion of too many factors influencing the experiment. We report a new method, orthogonal design-direct analysis, which has been confirmed to be scientific and easy to follow by many times of PCR.
出处
《湖南医科大学学报》
CSCD
1998年第4期403-404,共2页
Bulletin of Hunan Medical University
基金
湖南省自然科学基金
关键词
正交设计
聚合酶链反应
优化
orthogonal design
polymerase chain reaction
method
