摘要
目的:探讨不同浓度的仙鹤草水提液对体外培养的人肺癌细胞系A549细胞增殖及凋亡的影响。方法:采用不同浓度的仙鹤草水提液分别作用于体外培养的人肺癌细胞A549细胞株24、48、72h后,MTT法观察药物对肺癌A549细胞生长的抑制效应;作用于细胞48h后倒置显微镜观察细胞形态学的改变;应用AO/EB荧光染色法,观察不同浓度仙鹤草水提液对细胞凋亡的影响;Annexin V/P I双染流式细胞仪检测经药物作用后A549细胞凋亡情况。结果:仙鹤草水提液可剂量依赖性地抑制A549细胞的增殖,以48h的增殖抑制作用最好;通过倒置显微镜可明显观察到细胞的形态学改变;细胞荧光染色出现了明显的凋亡细胞,表现为细胞皱缩、核染色质浓缩、核碎裂等凋亡特征性的形态学改变;流式细胞仪检测结果显示,仙鹤草水提液可明显诱导细胞凋亡。结论:仙鹤草水提液可对人肺癌细胞系A549细胞具有显著的体外增殖抑制作用,其主要作用可能与诱导细胞凋亡有关。
Objective:To explore the effects of Agrimonia pilosa Ledeb water extract on proliferation and apoptosis of human lung cancer A549 cell line..Methods: MTT assay was used to observe the anti-proliferation effect of Agrimonia pilosa Ledeb water extract on A549 cells for 24h,48h,72h;Morphological changes were observed under optical microscopy;AO/EB fluorescence double-dye technology was performed to observe the apoptosis of cells treated for 48h in fluorescence microscope;The apoptosis rates of A549 cells treated by Agrimonia pilosa Ledeb. water extract for 24h were determined by flow cytometry (FCM).Results:Agrimonia pilosa Ledeb. water extract inhibited the proliferation of A549 cells in a dose-dependent manner.And the best proliferation was after being treated for 48h; Morphological changes were dramatical under optical microscopy;In experiment group appeared yellow or chrysoidine fluorescence ,cont racted , beaded and crescent and the number of apoptotic cells increased;FCM analysis showed that cells were induced apoptosis dose-dependently.Conclusion:Agrimonia pilosa Ledeb.water extract has significant antitumor activity in vitro.The potential mechanism of anti-proliferation effect caused by Agrimonia pilosa Ledeb. water extract may be cell apoptosis.
出处
《中医药信息》
2009年第5期33-35,共3页
Information on Traditional Chinese Medicine
基金
国家十一五支撑计划(2006BAI11B08)
