荧光素二聚体荧光探针同步荧光法测定阿米卡星

Determination of Amikacin by Synchronous Fluorescence Technique with Fluorescein Dimer as a Probe

基于阿米卡星（AMK）能增强荧光素二聚体的荧光信号，建立了以荧光素二聚体荧光探针同步荧光光谱技术测定AMK的新方法．结果表明，在△λ=30nm，最优条件下，测定AMK的线性范围为0．5～20．0μg／mL，检出限（LOD）为0．51μg／ml．用于实际样品分析，测定回收率为96．9％-104．5％，相对标准偏差（RSD）为3．7％～5．3％．

Base on the signal of fluorescein Dimer can be enhanced by Amikacin, A new method that taking Fluorescein Dimer as a fluorescence probe to determine Amikacin was built .The result showed that under the optimal condition of △k=30pm, good calibration curves of the Amikacin were obtained in the range of 0.5-20.0μg/mL with detection limits （LOD） of 0.51μg/ml. Applied to the commercial samples, this method was adaptable. And the recoveries ranged from 96.9% to 104.5% and RSD from 3.7% to 5.3%.