东宝肝泰片防治大鼠酒精性脂肪肝的作用及机制研究

Action Mechanism of Dongbaogantai Tablet in the Prevention and Treatment of Alcoholic Fatty Liver in Rats

目的:研究东宝肝泰片对酒精性脂肪肝(AFL)模型大鼠的防治作用及机制。方法:取SD大鼠随机分成正常组、模型组和东宝肝泰片组,后2组用白酒灌胃和高脂饲料喂养共8周以复制大鼠AFL模型,同时分别给予生理盐水和东宝肝泰片灌胃8周,测定各组大鼠血清甘油三酯(TG)、胆固醇(CHO)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平,天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)活性,肝组织中丙二醛(MDA)、游离脂肪酸(NEFA)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性,光镜下观察大鼠肝脏的病理变化。结果:与模型组比较,东宝肝泰片组TG、CHO、LDL-C、AST、ALT、MDA、NEFA水平显著降低(P<0.05),HDL-C、SOD、GSH-PX水平显著升高(P<0.05),其肝脏病理与正常组相似。结论:东宝肝泰片可通过减轻脂质过氧化反应有效防治AFL。

OBJECTIVE： To investigate the mechanism of the prevention and treatment effects of dongbaogantai tablet （DT） on rats with alcoholic fatty liver （AFL） . METHODS： SD rats were randomly divided into normal control group, model group and DT group. The model group and DT group were administered intragastrically with alcohol and fed with high fat diet for 8 weeks to replicate AFL model, meanwhile the model group were administered intragastrically with normal saline and DT group with DT synchronously for 8 weeks. Levels of TG, CHO, LDL- C, HDL - C, AST and ALT in serum, and MDA, NEFA, SOD and GSH- PX in hepatic tissue were detected and the pathological changes of liver were observed under light microscope. RESULTS： In DT group compared with model group, serum levels of TG, CHO, LDL - C, AST, ALT, MDA and NEFA were significantly down - regulated （P 〈0.05）, but levels of HDL - C, SOD and GSH - PX were significantly up - regulated （P〈0.05）; however, the pathological changes of liver were similar to those in normal control group. CONCLUSION： DT can effectively prevent and treat AFL by reducing the lipid peroxidation.