半夏内生菌的分离与初步鉴定

Isolation and identification of endophytes from Pinellia

目的:探讨半夏内生菌类群的多样性。方法:采用平板分离法分离半夏的内生真菌、细菌、放线菌,同时采用点植法、插片法和印片法对分离真菌菌株进行分类鉴定。结果:从多年生和一年生的半夏根茎叶样品中共获得内生菌84株,其中内生真菌48株、细菌31株、放线菌5株,内生真菌经形态观察分类鉴定为4科、15属。结论:半夏内生菌多样性丰富,同时不同部位内生菌的数量、种类及分布存在差异。

Objective： To investigate the varieties of entophytes in different parts of Pinellia ternata. Method： The solidified plates were applied for the isolation of the endophytes, and three methods were used for the identification of endophytic fungi. Result ： Eighty four strains of the entophytes were isolated from the P. ternata collected from 3 habitations. Endophytic fungi were morphologically identified belonging to 15 genera, 4 families. Conclusion： It indicated that the entophytes in P. ternata were diversity and rich, and there were some differences at quantity and species in different organs of P. ternata.