灵芝三萜类化学成分指纹图谱研究

Chromatographic fingerprints of triterpenoid constituents of Ganoderma lucidum

目的:建立灵芝药材三萜类化学成分高效液相(HPLC)指纹图谱,并以此评价灵芝药材的质量。方法:采用Dia-monsil C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈和0.8%高氯酸水溶液,梯度洗脱,流速0.9 mL.min-1,检测波长254 nm,柱温为室温。灵芝药材HPLC指纹图谱经指纹图谱系统解决方案软件(ChromafingerTM)生成共有模式,并进行相似度分析。结果:赤芝HPLC指纹图谱共有18个特征峰,其中6个化学成分通过对照品得到指认。赤芝与紫芝HPLC指纹图谱相差较大。结论:该方法为综合评价灵芝的质量提供了科学的依据。

Objective： To establish chromatographic fingerprints of triterpenoid compounds in ganoderma RP-HPLC for the quality control of ganoderma. Method ： HPLC fingerprints were established. The chromatographic conditions were as follows ： the separation was performed on a Diamonsil C18 column （4. 6 mm × 250 mm, 5 μm） , using a gradient elution with acetonitrile-water containing 0. 8% perchloric acid as the mobile phases. The flow rate was 0. 9 mL ·min^-1, the detection wavelength was 254 nm and the temperature of column was at ambient temperature. The common patterns of HPTLC and HPLC fingerprints were separately obtained through ＂Chromafinger＂ solution software, and quality assessments were analyzed by similarity. Result： The HPLC fingerprint pattern of G. lucidum consists of 18 peaks, among them 6 peaks were identified by chemical reference substances. Conclusion： This study provided theory and experimental data in order to comprehensively evaluate the quality of ganoderma.