摘要
目的:研究三七对大鼠肝组织内细胞色素P450(cytochrome P450,CYP)和谷胱甘肽转移酶(glutathioneS-trans-ferase,GST)基因表达的影响。方法:雄性SD大鼠,三七2,4 g.kg-1,每日1次连续灌胃14 d,用逆转录-实时定量PCR法检测肝组织内的CYP1A1,CYP1A2,CYP2B1,CYP2E1,CYP3A1,CYP4A1基因和GST-m1,GST-pi基因的表达情况。结果:三七不影响肝组织内CYP1A1,CYP1A2,CYP2E1,CYP3A1,GSTm1和GST-pi基因的表达,2,4 g.kg-1的三七可明显抑制CYP2B1基因(0.48倍,P<0.05和0.61倍,P<0.05)和CYP4A1基因(0.69倍和0.51倍)的表达。结论:三七可选择性地抑制大鼠肝组织内CYP2B1和CYP4A1的基因表达,这可能是三七的作用机制之一。三七对CYP1A1,CYP1A2,CYP2E1和CYP3A1的基因表达没有影响,提示三七与以上主要药物代谢酶代谢的药物合用时在肝内不会产生代谢性药物相互作用。
Objective： To examine the effects of Panax notoginseng on the expression of cytochrome P450 （CYP） genes and glutathione S-trans-ferase （GST） genes in liver tissues of male SD rats. Method： Rats were administered P. notoginseng 2 or 4 g · kg^-1 bw/d by garage daily for 14 days. The levels of gene expression of CYP1A1, CYP1A2, CYP2B1, CYP2E1, CYP3A1, CYP4A1, and GSTml, GST-pi were examined by quantitative real-time reverse-transcription polymerase chain reaction （ quantitative real time- RT-PCR） assays. Result： The expression of CYP1A1, CYP1A2, CYP2E1, CYP3A1, GSTml and GST-pi genes was not changed by 2 or 4 g · kg^-1 P. notoginseng treatment, But P. notoginseng significantly inhibited mRNA expressions of CYP2B1 （0. 48-fold, P 〈 0. 05, and 0. 61-fold, P 〈0. 05, respectively） and CYP 4A1 （0. 69-fold, and 0. 51-fold, respectively）. Conclusion： P. notoginseng had a special inhibitory selectivity on the expression of CYP 2B1 and CYP4A1 genes in liver tissues of rats, which indicated it may be one of the mechanisms of actions of P. notoginseng. P. notoginseng had no effects on the expressions of CYP1A1, CYP1A2, CYP2E1 and CYP3A1 genes, which suggested when P. notoginseng co-administrated with those drugs metabolized by the above major metabolizing enzymes in liver, metabolic herb-drug interactions would not be happened.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第18期2390-2393,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30660228)
广西科学基金项目(桂科基0731058)
