标记抗人肺癌单抗超顺磁性氧化铁粒子的磁共振免疫显像实验

The Experimental Study of Magnetic Resonance Immunoimaging Using Anti human Lung Cancer Monoclonal Antibodies Coated Superparamagnetic Iron Oxied Nanoparticle

目的：探讨标记抗人肺癌单抗的超顺磁性氧化铁微粒（ＳＰＩＯ）进行磁共振免疫显像的可能性。材料与方法：将采用Ｆｅ（Ⅲ）与Ｆｅ（Ⅱ）混合液在碱性条件下制备ＳＰＩＯ微粒；将粒径４．５ｎｍ±３．４ｎｍ的ＳＰＩＯ微粒标记到抗人肺癌单抗（ＭｃＡｂｓ）上；观察静注ＳＰＩＯ—ＭｃＡｂｓ定向改变载瘤裸鼠（ｎ＝４）ＭＲ信号强度的可能性。结果：ＳＰＩＯ—ＭｃＡｂｓＭＲ成像能有效地降低全部瘤靶的ＭＲ信号。

Objective: To investigate the possibility of MR immunoimaging using monoclonal antibodies against human lung cancer (McAbs) labelled with superparamagnetic iron oxide nanoparticle (SPIO).Materials and Methods:SPIO with a mean diameter of 4.5nm±3.4nm, was synthesized by reaction of Fe(Ⅲ) and Fe(Ⅱ) with dextran under the alkaline condition. The McAbs coupled convalently to SPIO was achieved with the potassium periodate method. Then the McAbs—SPIO was injected intravenously in nude mice hosted human lung cancer (n=4).Results:The MR contrast agent of SPIO—McAbs can selectively reduce the signal intensities of the tumor targets.Conclusion: The data indicate MR immunoimaging may become a new approach for specific imaging of tumors.