八角挥发油成分分析与抑菌活性研究

Studies on the chemical components and antimicrobial activities of volatile oil from Illicium verum Hook.f.

研究水蒸汽蒸馏法提取的八角挥发油的化学成分组成和抑菌活性。结果表明,八角挥发油的提取率为6.13%,从八角挥发油中分离鉴定出24种化合物,占挥发油总量的95.40%,其主要化学成分为反式大茴香脑和对烯丙基茴香醚,分别占76.93%和10.22%;挥发油对多种菌株有抑菌效果,对金黄色葡萄球菌、大肠杆菌、枯草杆菌、黑曲霉、黄曲霉和桔青霉的最小抑菌浓度分别为:10,20,20,0.625,2.5,5 mL/L。说明八角挥发油具有广谱的抗菌性,对霉菌的抑菌效果较好。

Study on the chemical components and antimicrobial activities of volatile oil from Illicium verum Hook. f. , which extracted by water steam distillation. The results showed that the extraction ratio of volatile oil from Illicium verum Hook. f. was 6.13% and 24 kind of compounds were identified from volatile oil, accounting for 95.40% of its total content. The major chemical compositions of volatile oil are E-anethole（76.93%） and p--Allylanisole （10.22%）. The volatile oil had inhibition to many bacterial species. The minimum inhibitory concentration to Staphylococus aureus, Escherichia coli, Bacillus subtilis, Aspergillus niger, Aspergillus flavus and Penicillium citrinum were respectivily： 10,20,20,0. 625,2.5,5 mL/L. It was concluded that the volatile Oil from Illicium verum Hook. f. has broad-spectrum antimicrobial activites, especially to the mould.