白首乌C_(21)甾苷元C_(11)α-羟化的两种微生物同步转化

C_(11) α-hydroxylation of C_(21) Steroidal Aglycones in Cynanchum Auriculatum by Microbial Simultaneously Transformation

目的对白首乌C21甾苷元告达庭甾苷元和开德甾苷元(Ⅱ)实现C11α-羟基化,要求副产物少,收率高。方法采用黑根霉(Rhizopus nigricans)和犁头霉(Absidia sp.)两种微生物在水-正丁醇双相体系中同步转化的方法,羟化反应中控制值pH在5.0～6.0,于30℃温度下振荡培养70h。结果产物经IR、MS、NMR分析确认是C11α-羟基化了的告达庭甾苷元(Ⅲ)和开德甾苷元(Ⅲ),副产物少,收率达70.1%。结论用黑根霉(Rhizopus nigricans)和犁头霉(Absidia sp.)两种微生物在水-正丁醇双相体系中同步转化的方法是实现C11α-羟基化的有效方法。

OBJECTIVE Two species of C21 steroidal aglycones of Caudatin steroidal aglycones and Kaider steroidal aglycones, which were extracted from Cynanchum auriculatum, could be C11 α-hydroxylated with low by-product content and high-yield objectives. METHODS Rhizopus nigricans and Absidia sp. were used to synchronized transformation and by shaking cultivation for 70 hours at 30℃ in water/butanol two-phase system with pH=5.0-6.0. RESULTS The products were confirmed to be C11 α-hydroxylated Caudatin steroidal aglycones and Kaider steroidal aglycones by IR, MS, and NMR. The results also indicated that by-product content was low and the yield of objectives was up to 70. 1%. CONCLUSIONS The method was high effective for C11 α-hydroxylation of C21 steroidal aglycones in water/butanol two-phase system by Rhizopus nigricans and Absidia sp..