5-氟尿嘧啶导致大鼠小肠功能损害

5-Fluorouracil induces damage of the small intestinal function in rats

目的：观察5-氟尿嘧啶（5－FU）对大鼠小肠功能的损害．方法：对照组动物（n=6）直接处死，5－FU组动物（n=24）经腹腔注射5－FU（150mg／kg）后，每8只大鼠分别于注射后第1，3，5日处死，检测大鼠小肠粘膜丙二醛含量，谷胱甘肽过氧化物酶，髓过氧化物酶和双糖酶活性，以及小肠对荧光异硫氰酸盐-葡聚糖4000的通透性．结果：5－FU组动物小肠粘膜丙二醇含量／μmol·g-1在注射后第1，3日（0．40±0．09，0．47±0．14）显著高于对照组（0．27±0．06，P＜0．05）；谷胱甘肽过氧化物酶活性／nmol·s-1·g-1在注射后第1日（109．0±70．2）显著低于对照组（397．9±148．2，P＜0．01）；髓过氧化物酶活性／nmol·s－1·g－1在注射后第1，3，5日（36．3±10．3，42．7±12．5，53．3±16．2）均显著高于对照组（7．8±3．7P〈O．01）；乳糖酶活性／μmol·s－1·g－1在注射后第5日（87．5±20．5），蔗糖酶和麦芽糖酶活性／μmol·s-1·g-1在注射后第3，5日（197．5±45．2，285．4±89．0；852．5±127．5，920．5±360．1）均显著低于对照组（17．0±62．5，438．6±163．7，1472．8±255．4，P〈0．01）；血浆中荧光异硫氰酸盐葡聚糖4000的浓度／mg·L-1在注射后第1，3，5日（7．86±1．22，6．98±1．01?

Aim: To investigate the damage of the small intestinal fu in rats induced by 5-fluorouracil (5-FU). Methods: Control group animals (n= 6) were directly sacrificed.5-FU group animals (n=24) were given a single intraperitoneal injection of 5-FU (150 mg/kg), every 8 rats weresacrificed on day 1, 3 and 5 after injection respectively forthe measurement of malondialdehyde content, glutathioneperoxidase activity, myeloperoxidase activity, disacchari-dase activity in intestinal mucosa and intestinal permeabilityto fluorescein isothiocyanate dextran 4000. Results: In 5FU group, malondialdehyde content/μmol · g-1 was signifi-cantly higher on day 1 and 3 (0. 40±0. 09,0. 47±0. 14 )af-ter injection than in contral group(0. 27 ± 0. 06,P<0. 05);Glutathione peroxidase activity/nmol·s-1· g-1 was significantly lower on day 1 (109. 0± 70. 2 ) than in control group(397. 9 ± 148. 2, P< 0.01); Myeloperoxidase activity/nmol· s-1·g-1 was significantly higher on day 1, 3 and 5 (36. 3±10. 3, 42. 7 ± 12. 5, 53. 3 ± 16. 2) than in contral group(7. 8 ± 3. 7,P<0.01 ); Lactase activity /μmol·s-1·g-1 onday 5 (87. 5 ± 20. 5 ), sucrase and maltase activites/μmol·s1·g-1 on day 3 and 5 (197. 5±45. 2,285. 4±89. 0;852. 5± 127. 5, 920. 5 ± 360.1 ) were significantly lower than incontral group (170. 0 ± 62. 5, 438. 6 ± 163. 7, 1472. 8 ±255. 4, P < 0. 01 ); Concentration of fluorescein isothio-cyanate dextran 4000 /mg·L-1 in plasma on day 1, 3 and 5(7. 86±1. 22, 6. 98±1.01, 7. 16±1. 07) was significantlyhigher than in contral group (5. 16±1. 09, P<0.01). Con-clusion: 5-FU may induce damage of the small intestinalfunction.