美国白蛾种群遗传分化的RAPD分析

RAPD analysis on genetic differentiation of Hyphantria cunea populations.

应用随机扩增多态性DNA(RAPD)技术,对9个不同地理种群美国白蛾进行了遗传多样性和UPMGA聚类分析。结果表明:25个RAPD引物共扩增191个位点,片段大小在200～2000bp,其中158个是多态位点,多态百分率为82.72%,遗传距离指数在0.2043～0.5108,遗传相似性系数在0.4892～0.7957;9个不同地理种群的美国白蛾可以分成3类:辽宁省和山东类群、北京和天津类群、美国类群,表明美国白蛾不同地理种群间的遗传分化与地理位置相关。

The genetic diversity and UPMGA clustering of nine Hyphantria cunea geographic＇ populations were analyzed by random amplified polymorphic DNA （RAPD） technique. A total of 191 bands were obtained from 25 effective primers, of which, 158 bands were polymorphic. accounting for 82. 72%. The genetic distance index of the populations was 0. 2043-0. 5108, and the similarity coefficient was 0. 4892-0. 7957. The nine populations could be divided into three groups, i. e. , Liaoning and Shandong group, Beijing and Tianjing group, and American group, suggesting that the genetic differentiation of H. cunea populations was correlated with geographical location.