N-三甲基壳聚糖对HaCat细胞膜电位的影响

Study on the Effect of N-trimethyl Chitosan on HaCat Cell Membrane Potential

目的:研究N-三甲基壳聚糖(TMC)对HaCat细胞细胞膜电位的影响及其与季胺化程度的关系。方法:合成季胺化程度分别为20%,40%及60%的TMC(即TMC_(20)、TMC_(40)、TMC_(60)),用流式细胞仪检测其对HaCaT细胞膜电位的影响。结果:与空白溶剂组比较,TMC能显著降低细胞膜电位,其中,TMC_(20)作用最明显,TMC_(40)组作用最微弱。结论:TMC可降低细胞膜电位,并与其季胺化程度密切相关,这可能是其发挥透皮吸收促进作用的机制之一。

Objective： To Study the effect of N-trimethyl chitosan （TMC） on HaCat cell membrane potential and the relationship with its degree of quaternization （DQ）. Method： TMCs with DQ of 20%, 40% and 60% （TMC20, TMC40, and TMC60 ） were synthesized. Membrane potentials of HaCaT cells were measured by flow cytometer. Result： Compared with the blank solvent, TMCs could decreased HaCaT cell membrane potential significantly, and the effect of TMC20 was the most significant while that of TMC40 was the mildest. Conclusion： TMCs could decrease the cell membrane potential and the effect had close relationship with their DQ.