固相萃取结合高效液相色谱法测定人全血中环孢素A的含量

Determination of Cyclosporin A in Plasma by SPE-HPLC

目的:建立固相萃取结合高效液相色谱法测定人全血中的环孢素A(CsA)含量,为CsA的血药浓度监测和药动学研究提供测定方法。方法:全血经固相萃取法处理,紫外检测,内标法定量。结果:CsA在50～500ng·ml^(-1)范围内线性关系良好(r=0.999 3)。高、中、低浓度的提取回收率均大于85%,日内、日间RSD均低于10%。结论:该法操作简便,灵敏度高,是一种较为理想的测量方法。

Objective ： To establish a method to determine CyclosporinA concentration in plasma by SPE-HPLC. Method： The CyclosporinA concentrations in plasma through solid-phase extraction were determined by HPLC method. Result： The linear range of CsA was 50 - 500ng·ml^- 1 （ r = 0.999 3 ） RSD of intra-day and inter-day were less than 10% and recovery of plasma coincided was well for pharmacokinetic study. Conclusion： The SPE-HPLC method to determine Cyclosporin A concentration is a good method.