双歧杆菌联合香菇多糖对肝硬化患者生理功能调节作用的研究

Bifidobacterium combined Lentinan regulates the physiological function of patients with cirrhosis

目的观察双歧杆菌联合香菇多糖治疗肝硬化患者的效果。方法肝硬化患者在接受常规保肝治疗的同时予口服双歧杆菌活菌胶囊联合香菇多糖胶囊。观察随访4周,治疗前后均抽取静脉血检测。采用常规方法检测肝功能;ELISA方法检测IFN-γ和IL-10水平的变化,流式细胞仪检测CD4/CD8细胞亚群的数量变化。结果临床一般状况明显好转,治疗前后部分肝功能指标明显改善。肝硬化组治疗前外周血CD4+T细胞数量和IFN-γ水平均显著低于正常对照组,治疗后二者明显升高,与治疗前相比差异有显著性。另外,与治疗前组相比,IL-10的水平治疗后明显降低。结论对于肝硬化患者,如果出现了明显的菌群失调伴随免疫功能低下者,宜采用微生态制剂联合免疫增强剂辅助治疗,临床效果较好。

Objective To observe the effect of Bifidobacterium combined Lentinan in patients with cirrhosis. Method Patients with cirrhosis were given Bifidobacterium combined Lentinan treatment while accepting conventional pro- tective therapy. Follow-up observations of 4 weeks were performed, and peripheral blood samples before and after treatment were detected. Liver function was detected by conventional methods; The levels of IFN-γ, and IL-10 were measured by ELISA; The number of CD4/CD8 was detected by FCAS. Result After treatment with Bifidbacterium combined Lentinan, the patients＇ general clinical state and some of their liver functions improved obviously. The number of CD4 ＋ T ceils and the level of IFN-γ, which were much lower than the normal control group, significantly increased after treatment and the differ- ence was significant （P 〈 0.05）, while the level of IL-10 decreased remarkably after treatment and the difference was sig- nificant （P 〈 0.01）. Conclusion To patients with cirrhosis who has clear dysbacteriosis with cellular immune dysfunc- tion, probiotics combined immuncenhancer could be considered as an adjunctive therapy in the treatment of chronic liver disease for a better clinical efficacy.