小剂量氨茶碱对支气管哮喘患者外周血CD4^+CD25^+调节性T细胞凋亡的影响

Apoptosis induced by low-dose aminophylline in asthmatic peripheral blood CD4^+CD25^+ T regulatory cells

目的观察小剂量氨茶碱对分离培养的健康人和支气管哮喘(简称哮喘)患者外周血CD4+CD25+调节性T细胞(Tregulatory cells,Treg)凋亡的影响。方法经密度梯度离心法、尼龙棉柱法、磁珠分离法分离出健康人和哮喘患者外周血CD4+CD25+Treg,分小剂量氨茶碱(1.13mg/L)、及空白组培养72h后,用流式细胞仪检测凋亡率变化。结果健康人外周血CD4+CD25+Treg的纯度为77.4%~92.3%,哮喘患者CD4+CD25+Treg的纯度为75.2%~93.8%。②CD4+CD25+Treg占外周血CD4+T细胞的比例在健康组为4.12%~7.98%,在哮喘组为4.51%~8.68%。两者差异无统计学意义。③小剂量氨茶碱均可以诱导健康组及哮喘组外周血CD4+CD25+Treg凋亡率增加(P<0.05)。结论小剂量氨茶碱(1.13mg/L)可能通过促进CD4+CD25+Treg凋亡来发挥免疫调节作用。

Objective To observe apoptosis induced by low-dose aminophylline in healthy and asthmatic human peripheral blood CD4^＋ CD25^＋ T regulatory cells（Treg）. Methods Peripheral CD4^＋ CD25^＋ Treg of healthy and bronchial asthma（asthma） was separated with Percoll （1. 073 g/ml） and harvested by filtration through nylon column and were separated after magnetic separation. Then, cells were cultured for 72 h while divided into low dose aminophylline （ 1.13 mg/L） and blank groups. The incidence rate of apoptosis was analyzed by flow cytometry. Results OThe separated rates of CD4^＋ CD25^＋ Treg were 77. 4%- 92.3% in healthy group,and were 75.2%-93.8% in asthmatic group. （2）The ratio of CD4^＋ CD25^＋ Treg in CD4^＋ T cells was 4.12% - 7.98% in health adults, and 4.51% -8.68% in asthma. There were no significant statistic differences between them. （3）Low dose aminophylline could upregulate the apoptosis ratios on CD4^＋ CD25^＋ Treg in health and asthma （ P 〈0.05）. Conclusions Low-dose aminophylline （1.13 mg/L）could educe immunomodulatory effect by inducing apoptosis in peripheral blood CD4^＋ CD25^＋ Treg.