摘要
目的:探讨色素上皮衍生因子(pigment epithelium-derived factor,PEDF)、肿瘤坏死因子(tumornecrosis factor,TNF-α)及脂联素等细胞因子在2型糖尿病视网膜病变(diabetic retinopathy,DR)发病机制中的作用。方法:选择经诊断为2型糖尿病患者无视网膜病变组35例;背景期DR组33例;增殖期DR组36例。同时选择40例健康正常人做对照组。应用酶联免疫分析法对PEDF,TNF-α及脂联素进行检测。结果:2型糖尿病视网膜病变患者的PEDF,TNF-α及脂联素表达显著下调(P<0.01),较正常对照组有显著性差异(P<0.01),且与病情的进程呈正相关。结论:PEDF是眼内有效的血管生成抑制剂,对保持正常眼组织透明无血管的状态有重要作用,TNF-α在介导炎症反应中起到了媒体作用,而脂联素具有促进眼内动脉重塑和新生内膜的生长作用。三者同时表达降低,可能提示在2型糖尿病视网膜病变患者免疫病理机制中的细胞活化模式,对2型糖尿病视网膜病变患者的免疫机制和指导治疗有指导意义。
AIM：To investigate the relation of pigment epithelium-derived factor（PEDF） ,tumor necrosis factor（TNF-α） and adiponectin with the pathogenesis of type 2 diabetic retinopathy（DR） .·METHODS：The study group consisted of 104 cases of type 2 diabetic patients. The diabetic patients group was divided into non-diabetic retinopathy（NDR） group（35 cases） ,non-proliferative diabetic retinopathy（NPDR） group（33 cases） ,and proliferative NDR group（36 cases） . Other 40 normal persons were as controls. The levels of PEDF,TNF-α and adiponectin were detected using enzyme-linked immunosorbent assay（ELISA） .·RESULTS：The expression levels of PEDF,TNF-α and adiponectin in patients with type 2 diabetic retinopathy significantly reduced（P〈0.01） ,compared with the normal control group were significantly different（P〈0.01） ,and were positively correlated with the process of type 2 diabetic retinopathy.·CONCLUSION：PEDF,an effective ocular angiogenesis inhibitor,plays an important role to maintain normal eye tissue transparent avascular status. TNF-α plays the media role of the mediated inflammatory response,adiponectin effects the promotion of ocular artery remodeling and neointimal growth. The simultaneous lower expression level of PEDF,TNF-α and adiponectin may reflect the mechanism of cell activation model with immune pathological in type 2 diabetic retinopathy,and has a guiding significance to immune mechanisms and treatment of type 2 diabetic retinopathy patients.
出处
《国际眼科杂志》
CAS
2009年第9期1695-1698,共4页
International Eye Science
