大鼠弥漫性轴索损伤后丘脑差异蛋白质表达

Differential proteomic analysis of thalamus in rats with diffuse axonal injury

目的:从蛋白质组角度探讨大鼠弥漫性轴索损伤后丘脑差异蛋白质表达的改变。方法:用固相pH梯度双向凝胶电泳分离空白对照组(n=10)及脑外伤组(n=15)大鼠的总蛋白质,经考马斯亮兰染色,PDQuest 7.0软件分析。结果:与空白对照组匹配,脑外伤组大鼠丘脑图像匹配率为95%,且重复性较好,其蛋白质主要分布在等电点pI 3～8,分子质量14.4～75.0 kD范围。与空白对照组比较,脑外伤组中蛋白质点表达上升的点有16个,下降的点有18个。结论:脑外伤可引起丘脑中蛋白质的改变。

Objective To examine the differential expression of protein of thalamus in rats with diffuse axonal injury.Methods Twenty-five rats were randomly divided into a normal group（n=10） and a trauma group（n=15）.Total proteins of brain trauma tissue and normal brain tissue were extracted separately,and then proteins were separated by two dimensional gel electrophoresis and stained with Coomassie brilliant blue.The differentially expressed protein spots were identified with biospectrometry.Images were analyzed by PDQuest 7.0.Results The distribution of protein spots in the trauma group was similar to that of the normal group,the matching rate was 95%,and the repeatability was good.Proteins were mainly displayed at pI 3~8,with relative molecular mass 14.4~75.0 kD.Compared with the normal group,16 spots of proteins increased and 18 spots of proteins decreased in the trauma group.Conclusion There is some difference in protein expression between the normal group and the trauma group.Brain trauma may lead to changes of proteins in the thalamus.