分次照射豚鼠腮腺早期放射性损伤的病理学观察

Pathohistology observation of the early radiation damage in guinea pig parotid glands after fractionated radiation

目的观察豚鼠在常规分割局部照射头颈部后腮腺早期放射损伤的病理变化。方法成年雄性豚鼠67只分别于照射剂量0,10,20,30,40,50Gy和50Gy后60天随机分为A～G7个组,给予常规分割γ射线局部照射头颈部,观察各组体重、腮腺重量、组织形态学改变、残存腮腺面积和炎症细胞计数。结果豚鼠体重在照射初期出现短暂体重下降之后逐渐增长。A～F组腮腺重量无显著差异(F=0.997,P=0.43),G组腺重较其他组显著增加(P<0.05);照后腮腺面积随照射剂量的增加而呈递减趋势,并出现不同程度的腺体结构改变和炎症细胞浸润,F、G组炎症细胞计数显著高于A～E组(P<0.05),F组与G组相当(t=0.17,P=0.87)。结论照射剂量与腮腺早期放射性损伤的程度呈正相关,放射诱导的炎症反应可能参与其中。

Objective To observe the tissue morphology of guinea-pig parotid glands after conventional fractionated radiation delivered to head-neck region.Methods Sixty-seven male guinea pigs were randomly divided into group A～G. Fractionated gamma-rays delivered to head-neck region was applied. According to different radiation dose and observation time,group A～G was set up at total dose 0,10,20,30,40,50Gy and 60 days after 50Gy respectively. The body weight,gland weight,histopathologic change,remaining gland areas and inflammatory cell counts of parotid gland were compared respectively.Results The body weight of guinea pigs increased gradually after transient weight loss in the initial stage of radiation. The gland weight of group G significantly increased compared with other groups （P〈0.05）,while there was no difference among group A～F （F=0.997,P=0.43）. The average parotid gland areas presented a decreasing trend with the irradiation dosage upgrade. For varying degrees of gland structural changes and inflammatory cell infiltration occurred after radiation. The inflammatory cell counts of group F and group G were significant higher than those of the other groups （P〈0.05）,while there was no difference between them （t=0.17,P=0.87）.Conclusion There is a positive correlation between irradiation dose and the level of early irradiation damage on parotid gland,and inflammatory reaction induced by irradiation maybe participate in it.