硝酸甘油对大鼠重症急性胰腺炎ET／NO、TXA2／PGI2的影响

The effect of nitriglycerin on ET/NO and TXA2/PGI2 in severe acute pancreatitis rats

目的探讨硝酸甘油对大鼠重症急性胰腺炎（SAP）ET／NO、TXA2／PGI2和胰腺病理形态学的影响。方法SD大鼠60只分为5组：A正常胰腺组，B、C、D和E组为实验组，胰胆管逆行注射5％牛磺胆酸钠制作SAP模型。C、D和E组分别静脉注射硝酸甘油0．5、1．0、2．0μg/（kg·min），A、B纽注射生理盐水。6h及12h后分别检测各组ET、NO、TXB2及6-keto—PGFIa值并进行胰腺病理标本评分。结果6h及12h时的标本数据分别显示，与B组比较，C、D和E组的胰腺病理评分、ET、ET／NO、TXB2、TXB2／6-keto—PGFIa比值都降低（P〈0．05）。结论硝酸甘油具有降低大鼠血液ET、ET／NO、TXA2、TXA2／PGI2值的作用，改善胰腺血液微循环，延缓大鼠重症急性胰腺炎的炎性病理变化的作用。

Objective To explore the effect of nitroglycerin on ET/NO, TXA2/PGI2 and pancreas pathomorphology changes in severe acute pancreatitis （SAP） rats. Methods Sixty SD rats were random divided into five groups, including control group （A group, n = 12） and experimental group（ B ,C ,D and E group, n = 12）. The SAP was induced by injection of 5% sodium taurocholate through retrograde common biliopancreatic ducts via duodenal papilla with epidural catheter. Group C, D and E were intravenously injected with nitroglycerin 0.5μg/kg/ min,1μg/kg/min and 21μg/kg/min in 30 min respectively, and group A and B was injected with Sodium Chloride 0. 5ml. The indexes of changed pathomorphology and ET/NO,TXB2/6-keto-PGFIa were determined at the 6th and 12th hour after operations, respectively. Results The specimen data of the 6th and 12th hour displayed that the indexes of changed pathomorphology, ET, ET/NO,TXB2, and TXB2/6-keto- PGFI, of the group C,D and E degraded respectively, compared to group B （ P 〈0.05）. Conclusion The nitroglycerin could degrade ET, ET/NO ,TXA2 and TXA2/PGI2 , improve the microcirculation of pancreas, and delay the pathological inflammation change in SAP rats.