摘要
目的研究不同工艺参数对赤灵芝醇溶性蛋白提取率的影响,为探索赤灵芝醇溶性蛋白的工业化生产提供参考。方法以赤灵芝为原料,采用乙醇提取醇溶性蛋白,并通过单因素实验和正交实验研究浸提时间、温度、pH、液料比及提取次数对醇溶性蛋白提取率的影响,确定赤灵芝醇溶性蛋白提取的最佳工艺。结果单因素实验结果表明,提取时间2h,料液比1:40,醇溶性蛋白含量最高。正交实验得出各因素对灵芝醇溶性蛋白提取率的影响程度大小依次为:pH值>料液比>提取温度>乙醇浓度,醇溶性蛋白提取的最佳工艺参数为:乙醇浓度60%,pH2.5,料液比1:40,提取温度60℃。结论通过单因素实验和正交实验确定了赤灵芝醇溶性蛋白的最佳提取工艺参数,为赤灵芝醇溶性蛋白的工业化生产提供了参考依据。
Objective The study was to investigate the effect of different process parameters to the yield of ethanol-soluble protein from Gnaoderma lucidum, aims to provide theoretical guidance for industry production of ethanol-soluble protein from Ganoderma lucidum.
Methods Ethanol-soluble protein was extracted from Ganoderma lucidum. The effects of extraction time, extraction temperature, pH, material-liquid ratio and extraction frequency on the yield of ethanol-soluble protein were studied with single factor and orthogonal tests. And then the optimum extraction technology for ethanol-soluble protein from Ganoderma lucidum were determined.
Results The results of single factor test showed that the yield of ethanol soluble protein from Ganoderma lucidum was the highest when material-liquid ratio was 1：40, extration time was 2 h. The effects of factors on yield of ethanol-soluble protein from Ganoderma lucidum from big to small in order was pH 〉 material-liquid ratio 〉 extraction temperature 〉 concentration of ethanol. The optimal condition of ethanol-soluble protein was as follows： concentration of ethanol 60%, pH 2.5, material-liquid ratio 1：40 and extration temperature 60 ℃.
Conclusion Ganoderma lucidum. Through single factor and orthogonal tests, we found an optimal condition on yield of ethanol-soluble lucidum, which provide a theoretical guidance for industry production of ethanol-soluble protein from protein from Ganoderma
出处
《中国医药生物技术》
CSCD
2009年第5期359-363,共5页
Chinese Medicinal Biotechnology
关键词
灵芝
正交试验
Ganoderma lucidum
Orthogonal test
