摘要
目的观察VitK3(维生素K3,vitaminK3)对HeLa细胞损伤过程中的自噬相关蛋白Beclin1、LC3-Ⅱ表达的影响。方法以HeLa细胞为研究对象,采用MTT检测细胞存活率,用Western blot方法检测自噬相关蛋白Beclin1、LC3-Ⅱ表达的变化和蛋白激酶B(PKB,Akt)/哺乳动物雷帕霉素靶蛋白(mTOR)、磷酸化-Akt/mTOR表达的变化。结果30μmol·L-1剂量以上的VitK3可明显抑制HeLa细胞的增殖(P<0.05)。VitK3作用12h后增加Beclin1和LC3-Ⅱ的蛋白表达水平(P<0.05),降低磷酸化的Akt/mTOR的蛋白表达水平(P<0.05)。结论VitK3能明显抑制HeLa细胞增殖并具有诱导HeLa细胞发生自噬的作用,其机制可能与下调mTOR信号有关。
Aim To observe the effect of Vit K3 on Beclin 1, LC3-Ⅱ protein expressions in HeLa cells injuried. Methods HeLa cells were used for in vitro cultrure study. The cell survival rate was examined by MTT asssy. Autophagy-related protein Beelint, LC3-Ⅱ and protein kinase B ( PKB, Akt), mammalian target of rapamycin ( roTOR ), Phospho-Akt, Phospho-mTOR protein expressions were tested by Western blot analysis. Results Vit K3 obviously inhibited the proliferation of HeLa cells ( ≥ 30μmol·L^-1, P 〈 0.05 ). An exposure of HeLa cells to Vit K3 12 h produced upregulation of Beclinl, LC3-Ⅱ protein levels and downregulation of phospho-Akt/mTOR protein levels (P 〈 0.05 ). Conclusion Vit K3 has an effect on inhibiting the cell proliferation and inducing autophagy of HeLa cells, which might be correlated with downregulation of mTOR pathway.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2009年第9期1173-1176,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30570687)