人类UGT2B17基因缺失的多重PCR分型方法

Multiplex PCR genotyping method for human UGT2B17 gene deletion

目的建立一种快速有效的人类UGT2B17基因缺失型的PCR分型方法。方法针对人类基因组UGT2B17基因缺失型和外显子序列设计引物,采用多重PCR法进行分型。结果成功建立了一种多重PCR基因分型方法,并以普通PCR法和测序法验证。对安徽皖南地区健康人群UGT2B17基因分布进行了初步分析。结论所建立的多重PCR方法为一种有效便捷的UGT2B17基因缺失分型方法。

Aim To establish a fast and efficient genotyping method for human UGT2B17 gene deletion polymorphism. Methods Specific primers were designed for the deletion and the exons of UGT2B17 respectively. Multiplex PCR methods were applied in UGT2B17 genotyping with the primers. Results A multiplex PCR method for UGT2B17genotyping was established successfully, confirmed by sequencing and common PCR reaction. The UGT2B17 gene distribution of healthy population in Wannan area was studied preliminarily. Conclusion Mutation screening of the UGT2B17 gene deletion using this multiplex PCR is demonstrated to be a convenient and efficient method to study the deletion genotype.