HPLC法测定陆英花和果实中齐墩果酸和熊果酸的含量

Determination of Ursolic Acid and Oleanolic Acid in Flowers and Fruits of Sambucus Chinensis Lindl. by HPLC

建立高效液相色谱法同时分离和测定陆英花和果实中齐墩果酸和熊果酸的含量。选用Kromasil C18色谱柱，流动相为甲醇：水：磷酸（90：10：0．2），检测波长210nm，流速0．8mL／min，柱温25℃。齐墩果酸进样量在n55—5．50μg，熊果酸进样量在1．02—10．20μg时呈现良好的线性关系，相关系数均为0．9999，齐墩果酸和熊果酸的平均回收率分别为99．3％（RSD为1．9％）和101．2％（RSD为2．3％）.本法具有准确、灵敏、数据可靠的优点，可用于陆英花和果实的齐墩果酸和熊果酸的分离与测定。

A method for determination of oleanolic acid and ursolic acid simultaneously in flowers and fruits of Sambucus Chinensis Lindl was established. HPLC was carried out on a Kromasil C18 column using MeOH ： H20 ： H3PO4 （ 90 ： 10 ： 0.2, with detection at 210 nm, flow rate 0.8 ml/min and column temperature 25 ℃. An average recovery of 99.3 % was obtained with a RSD of 1.9 % in the range of 0.55-5.50 μg for oleanolic acid, and an average recovery of 101.2 % was obtained with a RSD of 2.3 % in the range of 1.02-10.20 μg for ursolic acid. There were both good linear correlations with the correlation coefficients of 0.9999.. The method is accurate, sensitive and the data is reliable, and it can be used for the isolation and determination of oleanohc acid and ursolic acid in flowers and fruits of Sambucus Chinensis Lindl.