摘要
目的观察转染外源性PTEN基因人胃癌细胞的生长情况及其对化疗药物敏感性的影响。方法人胃癌BGC823细胞转染PEAK8空载质粒和PEAK8-PTEN质粒,稳定表达后予足叶乙甙和阿霉素干预;RT-PCR法检测PTEN mRNA表达,MTT法检测细胞生长活力,流式细胞术分析细胞周期。结果转染PEAK8-PTEN质粒的BGC823细胞PTEN mRNA强表达,明显高于转染空载质粒和未转染细胞;PTEN转染后肿瘤细胞存活率下降(P<0.05),联合化疗药细胞存活率更低(P<0.01);转染PTEN加两种化疗药处理后均出现G2/M为0,细胞阻断于S期。结论转染PTEN基因的人胃癌BGC823细胞生长受抑制,对化疗药敏感性增加。
Objective To observe the growth and susceptibility to chemical therapy drug of gastric carcinoma cell transfected exogenous PTEN gene. Methods PEAK8 and PEAK8-PTEN plasmid were transferred into gastric carcinoma cell line BGC823 . The stable expression cell line was interfered by etoposide and doxorubicin hydrochloride. The expression of target genes was detected by RT-PCR. Cell viability was determined by MTT assay. Cell cycle analysis was determined by flow cytometry. Results The expression of PTEN was verified in group transfected with PEAK8-PTEN plasmid, but not in PEAK8 group and the control group . The groups transfected with PTEN had much lower cell viability than corresponding ones（ P 〈 0.05 ） , especially in PEAK8-PTEN group interfered by chemical drug meanwhile （P 〈 0.01 ） . PTEN genc transfection could block cell cycle before S stage. Conclusion PTEN gene transfection could suppress the growth of gastric carcinoma cell line BGC823 and enhance its susceptibility to chemical therapy drug in vitn.
出处
《山东医药》
CAS
北大核心
2009年第34期1-3,共3页
Shandong Medical Journal
