肝外胆管癌组织中HIF-1α、VEGF的表达及其相关性

Expression and relationship of HIF-1α and VEGF in extrahepatic cholangiocarcinoma

目的观察肝外胆管癌组织中缺氧诱导因子-1α(HIF-1α)与血管内皮生长因子(VEGF)的表达及其相关性。方法采用免疫组化SP法检测50例肝外胆管癌及20例正常肝外胆管组织中的HIF-1α及VEGF。结果肝外胆管癌组织中HIF-1α、VEGF表达均明显高于正常肝外胆管组织(P<0.01);二者表达与肝外胆管癌淋巴结转移有关(P<0.05);在肝外胆管癌组织中HIF-1α、VEGF的表达呈正相关(r=0.467,P=0.01)。结论HIF-1α、VEGF在肝外胆管癌组织中均呈高表达,两者对肝外胆管癌的发生、发展及转移有协同作用。

Objective To observe the expression of hypoxia-inducible factor 1 alpha（HIF-1α） and vascular endothelial growth factor（VEGF） in extrahepatic cholangiocarcinoma tissue, and investigate their relationship. Methods The expression of HIF-1α and VEGF were detected by immunohistochemistry SP in 50 cases of extrahepatic cholangiocarcinoma and 20 cases of normal extrahepatic bile duct tissue. Results The expression of HIF-1α and VEGF in extrahepatic cholangiocarcinoma tissue were obviously higher than in normal extrahepatic bile duct tissue（ P 〈 0.01 ） ,The expression of HIF-1α and VEGF had no significant relationship with sex,age,tumor size,TNM clinical stage and the degree of differentiation,but which is related to lymph node metastasis（ P 〈 0.05 ） ;The expression of HIF-1α and VEGF in extrahepatic cholangiocarcinoma tissue were positive correlation（r = 0.467, P = 0.01 ）. Conclusion HIF-1α and VEGF are high expression in extrahepatic cholangiocareinoma tissue, both have synergistic effect in the occurrence of extrahepatic cholangiocarcinoma, development and transfer.