尼莫地平、环磷酰胺、SOD联合治疗大鼠脑缺血再灌注损伤疗效分析

Neuroprotective effects of combined nimodipine with cyclophos phamide and superoxide dismutase on cerebral ischemia-reperfusion in rats

目的研究尼莫地平、环磷酰胺和超氧化物歧化酶(SOD)联合治疗对大鼠脑缺血再灌注损伤的保护作用。方法93只成年雄性Wistar大鼠随机分成:单药治疗组(A、B、组)、二联药物治疗组(D、E、F组)、三联药物治疗组(G组)及对照组(H组)。线栓法制作大鼠MCAO模型,再灌注前20 min、再灌注后12 h和36 h尾静脉给药。缺血4 h再灌注48 h后计算大鼠存活率、神经功能评分和脑梗死体积。结果与对照组相比,联合药物治疗组的大鼠存活率显著增加,神经功能缺损明显减少,脑梗死体积明显减少(P<0.05或P<0.01)。结论SOD、尼莫地平、环磷酰胺联合治疗抗脑缺血再灌注损伤效果显著,疗效优于两种药物联合治疗与单药治疗。

Objective To investigate the effects of combination therapy of nimodipine,cyclophosphamide and superoxide dismutase on reperfusion injury after focal cerebral ischemia in rats.Methods Ninety-three adult male Wistar rats were randomly divided into 8 groups： single-drug treatment group（A,B,C group）,two-drug treatment group（D,E,F group）,triple drug treatment group（G group） and the control group（H group）.A rat model of middle cerebral artery occlusion（MCAO） was induced by the intraluminal suture method. Reperfusion was performed 4 h after cerebral ischemia. At 20 minuters before and 12 h, 36 h after reperfusion, the medications （nimodipine, cyelophosphamide and superoxide dismutase were dissolved in 1.5 ml isotonic saline） were injected slowly into the tail veins of rats. The survival rate of rats was calculated. Neurological deficit score and the volume of cerebral infarction were measured 48 h after cerebral ischemiareperfusion. Results Compared with the control group, the groups of combined medicines increased in survival rate and decreased in neurological deficits and the volumes of cerebral infarction （P 〈 0.01 or P 〈 0.05 ）. Conclusions The neuroprotective effects of combination of nimodipine with cyclophosphamide and superoxide dismutase on focal cerebral ischemiareperfusion injury in rats were better than that of other medicines.