马尾松优良菌根真菌(S.l)液培条件的研究

A Study on the Liquid Cultural Conditions for a Mycorrhiza Fungi (S.l) with Pinus massoniana

作者经过对褐环乳牛肝菌［Ｓｕｉｌｕｓｌｕｔｅｕｓ（Ｌ．ｅｘＦｒ．）Ｇｒａｙ］的一系列研究，业已证明它是我国亚热带地区特有树种马尾松（ＰｉｎｕｓｍａｓｏｎｉａｎａＬａｍｂ．）共生的优良菌根真菌。它具有生长快速、繁殖容易、拮抗性强、适生范围广、制剂简便、...

Suillus luteus (L.ex Fr.) is a superior mycorrhiza fungi being symbiosis with Pinus massoniana in the subtropical area of China. The fungi is widely distributed and has been effectively applied to the pine. In order to examine the liquid cultural conditions of the S. luteus, the temperature,pH,carbon sources,nitrogen sources and C/N for the growth and the propagation of the fungi were tested in the study. The results showed that for the growth and propagation of the hyphae of the strain S.l, the suitable temperature range was 20～30 ℃, the optimum 25 ℃;the suitable pH was 3～8,the optimum 4～4.5. All the carbon sources tested can be absorbed (glucose,fructose,saccharose,amylomaltose,ethanol,mannitol, soluble starch,dextrin) but the optimum was soluble starch;all the nitrogen sources [(NH 4) 2SO 4,(NH 4) 2HPO 4,NH 4NO 3,KNO 3,Urea,tartaric acid,aminoacetic acid,glutamic acid],except inorganic nitrogen (KNO 3),can be absorbed but the optimum was glutamic acid. A wider range of C/N was effective but the optimum was 20∶1.