摘要
为确定ＣＨＬ细胞为测试靶细胞时ＦＡＤＵ的较佳实验参数，采用变化解旋温度及解旋液碱浓度，并根据Ｂｉｒｎｂｏｉｍ等的判断标准，结果表明解旋温度先０℃３０ｍｉｎ后１５℃６０ｍｉｎ；解旋液ＮａＯＨ浓度０４Ｎ效果较佳。并以此为条件把该法应用于检测过氧化氢（Ｈ２Ｏ２）诱导的ＤＮＡ过氧化损伤－适应及抗过氧化作用。
To make certaion stable experimental parameters about FADU in detection of CHL DNA breaks, temperature of DNA unwinding and concentration of unwinding alkaline solution NaOH were modified through FADU on CHL cells. According to Birnboim's criterion, unwinding temperature first 0℃/30 min→then 5℃/60 min and NaOH 0.4N were selected as better conditions. The reliability of these experimental parameters was established by detection of peroxidative adaptive response and antiperoxidation on CHL DNA breaks induced by H 2O 2 with the improved FADU.
出处
《癌变．畸变．突变》
CAS
CSCD
1998年第5期278-281,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金
卫生部科学研究基金
关键词
CHL细胞
DNA
解螺旋荧光分析
解旋温度
CHL cells
FADU
temperature of unwinding
concentration of unwinding alkaline solution
peroxidative adaptation
antiperoxidation
