低剂量γ-射线联合香烟烟雾对人淋巴细胞及小鼠骨髓细胞微核作用的研究

STUDY ON EFFECTS OF THE LOW DOSE GAMMA RADIATION COMBINING CIGARETTE SMOKING ON MICRONUCLEUS OF HUMAN LYMPHOCYTES AND MICE BONE MARROW

本文应用体外培养人淋巴细胞和小鼠骨髓细胞微核的检测，对低剂量γ射线与香烟的复合诱变作用进行了研究。结果表明：香烟（０８～１支／ｍｌ）与低剂量γ射线（０．２５１０Ｇｙ）均显著诱发培养人淋巴细胞微核的形成（Ｐ＜００１），并呈明显的剂量效应；低剂量γ射线联合香烟处理培养人淋巴细胞，诱发微核率显著高于对照组。小鼠体内实验也表明了低剂γ射线联合高浓度香烟对小鼠骨髓细胞微核有明显诱发作用。

Through observations on the micronucleus formation in human lymphocytes in vitro and bone marrow of mice, the induced mutagenesis effects of low dose gamma radiation combining cigarette smoking were studied. Cigarette smoking (0.8￣1cigarette/ml) and low dose gamma radiation (0.25￣1.0Gy) induced signficantly micronulei formation in human lymphocytes(P<0.01) respectively and the significant dose response was found. The significant higher rate of micronuleus than that of control group was observed when low dose gamma radiation combining cigarette smoking was used to treat the human lymphocytes. When low dose gamma radiation combining high concentration cigarette smokeing (1.5cigarette/ml) was used to tread the mice, the significant effects of induction and interaction on the micronucleus formation of mice bone marrow were also observed. The results indicated that hereditary substance aberrations would be enhanced when low dose gamma radiation combined cigarette smoking.