气管内应用脂质体前列腺素E_1对油酸致大鼠急性肺损伤的保护作用

Protective effect of intratracheally administered liposomal prostaglandin E 1 on oleic acidinduced lung injury in rats

目的：探讨气管内应用脂质体携载的前列腺素Ｅ１（ＬｉｐＰＧＥ１）对油酸致大鼠急性肺损伤的保护作用及其机制。方法：Ｗｉｓｔａｒ大鼠４０只，随机均分为５组：正常对照组、油酸致伤组、ＬｉｐＰＧＥ１组、游离前列腺素Ｅ１（ＦＰＧＥ１）组和空白脂质体（ＰＬｉｐ）组。观察肺系数、肺湿重／干重比、肺组织碱性磷酸酶（ＡＬＰ）、血管紧张素转换酶（ＡＣＥ）、髓过氧化酶（ＭＰＯ）的活性和丙二醛（ＭＤＡ）含量的变化。结果：与油酸损伤组比较，ＬｉｐＰＧＥ１组的肺系数、肺组织湿重／干重比与ＭＰＯ活性、ＭＤＡ水平明显降低，而ＡＬＰ与ＡＣＥ活性显著提高；ＬｉｐＰＧＥ１的作用显著优于ＦＰＧＥ１；ＰＬｉｐ无效。结论：经气管内给予ＬｉｐＰＧＥ１对油酸引起的大鼠急性肺损伤有良好的保护作用，其机制可能与抑制肺内中性粒细胞的聚集与抗氧自由基所造成的损伤有关。

Objective:To observe protective effect of intratracheally administered liposomal prostaglandin E 1(PGE 1) on oleic acidinduced lung injury and its potential mechanisms.Methods:Forty Wistar rats were randomly divided into 5 groups:normal control (NC) group,oleic acidinduced injury group (OA group),liposomePGE 1 (LipPGE 1)group,free PGE 1 (FPGE 1)group,and plain liposome (PLip)group,respectively.The pulmonary coefficient,lung wet weight/dry weight ratio,alkaline phosphatase (ALP),angiotensinconverting enzyme (ACE) as well as myeloperoxidase (MPO) activities,and lung malondialdehyde (MDA) contents were measured in those animals.Results:The pulmonary coefficient,lung wet weight/dry weight ratio,MPO and MDA levels were markedly lower,but ALP and ACE activities were significantly higher in LipPGE 1 group than those in OA group.Treatment with LipPGE 1 had obviously better effect on acute lung injury compared to FPGE 1.Conclusions:The results suggested that LipPGE 1 administered intratracheally has protective effects on oleic acidinduced lung injury in rats,and it may be attributable to the inhibition of pulmonary neutrophil accumulation and the decrease of oxidative damage.