ELISA法检测乳制品中的三聚氰胺质量浓度

Determination of melamine in dairy products by ELISA

建立酶联免疫吸附法（ELISA）检测乳制品中三聚氰胺的残留量。结果表明，乳制品中的三聚氰胺质量浓度在20～500μg／L范围内呈线性关系，相关系数R^2=0．9989；最低检出限为20μg/L；加标回收率：液70％-92％，固态奶94％-108％；相对标准偏差（RSD）小于13％；与结构类似物无交叉反应。该方法简便、快速、准确，能够满足快速筛检要求。

To establish an enzyme-linked immunosorbent assay （ELISA） for the determination of melamine in dairy products. The results show that the linearity was within 20-500 μg/L, R^2=0.9989. The recoveries of melamine in liquid milk and powder were 70% - 92% and 94% - 108% respectively, and the detection limit was 20μg/L. The relative standard deviation （RSD） was within 13%. There was no cross-reactivity with structural analogues of melamine. This method is simple, rapid, precise and can meet the repid screen detect requirements.