摘要
目的观察Ｒｂ蛋白在胃癌中的表达及与浸润、转移和临床分期的关系。方法用链霉菌抗生物素蛋白—过氧化酶连接法检测Ｒｂ蛋白在正常胃粘膜、不典型增生、胃癌中的表达。结果Ｒｂ蛋白表达率，正常胃粘膜１００％（１０／１０），明显高于不典型增生８０％（４８／６０）（Ｐ＜０．０５）和胃癌６０％（７２／１２０）（Ｐ＜０．０１）；不典型增生与胃癌有显著性差异（Ｐ＜０．０１），但中度８０％（１６／２０）、重度７５％（１２／２０）却无显著性；高分化腺癌６８％（２３／３４）、低分化腺癌５８％（２９／５０）、未分化癌６４％（９／１４）、粘液癌５０％（６／１２）、印戒细胞癌５０％（５／１０），各型之间无显著差异；浸润浅肌层者７５％（３０／４８）较浸润深肌层及全层者５０％（３６／７２）（Ｐ＜０．０１），未转移者６５％（２６／４０）较转移者３０％（６／２０）（Ｐ＜０．０１），临床Ⅳ期４０％（６／１５）较Ⅰ期８２％（９／１１）、Ⅱ期７１％（３０／４２）（Ｐ＜０．０５）皆具有显著性差异。结论Ｒｂ缺失与胃癌发生、浸润与转移及临床分期有关，从分子水平证实中度、重度不典型增生是癌前病变，检测Ｒｂ蛋白有助于胃癌的诊断。
Objective: To investigate the relation of Rb protein expression to gastric carcinogenesis, depth of invasion, lymph node metastasis and clinical staging in gastric carcinoma. Methods: The expression of Rb protein was examined by streptavidin-peroxidase conjegred method (SP) in normal gastric mucosa, dysplasia and gastric carcinoma. Results: The frequency of Rb protein expressionin 100% (10/10) of normal gastric mucosa was remarkably higher than that in 80% (48/60) of dysplasia ((P<0.05) and in 60% (72/120) of gastric carcinoma (P<0.01), and that in gastric carcinoma was significantly lower than in dysplasia (P<0.01), but the difference between gastric carcinoma and moderate or severe dysplasia was not significant.Rb positivity was 68%, 58%, 64%, 50% and 50% in well-differentiated, poorly-differentiated, undifferentiated, mucoid carcinoma and signet-ring cell carcinoma respectively (P>0.05). Rb expression was related to depth of invasion (P<0.01), and a significant difference was found between non-metastasis (65%) and lymph node metastasis (30) (P<0.01) and between stage Ⅳ (40%) and Ⅰ (82%), Ⅱ(71%) (P<0.05). Conclusions: It is suggested that loss of Rb expression is related to gastric carcinogensis, depth of invasion, lymph node metasis and clinical staging, that moderate and severe dysplasia are precancerous lesion, and that examing Rb protein is helpful for diagnosis and evaluation of biologic behaviour and prognosis in gastric carcinoma.
基金
湖南省卫生厅"八.五"重点项目科研基金
