灵龟八法对肾阳虚模型豚鼠自由基代谢及血液流变学的影响

Effect of Lingguibafa on free radical metabolism and hemorheology in the kidney-yang deficiency model guinea pigs

目的观察运用灵龟八法按时开穴灸对肾阳虚模型豚鼠的影响和作用机制。方法以健康成年豚鼠为研究对象,随机分为肾阳虚模型灵龟八法预防组、肾阳虚模型命门灸预防组、肾阳虚模型灵龟八法治疗组、肾阳虚模型命门灸治疗组、肾阳虚模型对照组等5组,以自由基代谢及血液流变学为客观指标,比较灵龟八法按时开穴灸、命门灸对肾阳虚模型豚鼠自由基代谢及血液流变学的影响差异。结果在造模的同时进行灵龟八法按时开穴灸治可以使豚鼠不出现肾阳虚征象,且其自由基代谢及血液流变学改变不明显;命门灸预防组没有出现明显的肾阳虚征象,自由基代谢改变不明显,但血液流变学中200及30s-1升高明显。在已出现肾阳虚征象,同时自由基代谢及血液流变有显著改变的肾阳虚模型豚鼠中,进行灵龟八法按时开穴灸治后肾阳虚征象消失,超氧化物歧化酶(SOD)、丙二醛(MDA)、200s-1基本恢复到造模前水平,30s-1无明显改变;而命门灸治疗组治疗后肾阳虚症状改善明显,MDA、200s-1基本恢复到造模前水平,SOD升高,但未恢复到造模前水平,30s-1无明显改变。结论灵龟八法对肾阳虚模型豚鼠具有明显的防治作用,其延缓衰老机理可能与调节自由基代谢及血液流变学密切相关;灵龟八法按时开穴灸对肾阳虚模型豚鼠的防治作用略优于命门灸。

Objective To observe the effect of Lingguibafa moxibustion on the kidney-yang deficiency model guinea pigs and its mechanism. Methods Healthy and adult guinea pigs were randomly divided into 5 kidney-yang deficiency model groups： Lingguibafa prevention, Mingmen （GV4） moxibustion prevention, Lingguibafa treatment, Mingmen moxibustion treatment and control groups. Free radical metabolism and hemorheology were observed. Results There were no signs of kidney-yang deficiency or significant changes in free radical metabolism and hemorheology with Lingguibafa moxibustion during making model process. In Mingmen moxibustion group, there were no obvious signs of kidney-yang deficiency or significant changes in free radical metabolism, but there showed remarkable increase in 200 and 30 s^- 1 with significant statistical values. After Lingguibafa moxibustion treatment, the symptoms of kidney-yang deficiency significantly disappeared in kidney-yang deficiency model guinea pigs with significant changes in free radical metabolism and hemorheology, and SOD, MDA, 200 s ^-1 had reverted to the normal level before modeling, but there wad no obvious changes in 30 s^ -1. While in Mingmen moxibustion group, the symptoms of kidney-yang deficiency significantly improved, MDA, 200 s^-1 had reverted to the normal level, SOD was inreased. And 30 s^-1 did not significantly change. Conclusions Lingguibafa has obvious preventing and treating effect on kidney-yang deficiency, which may be related to the regulation of free radical metabolism and hemorheology. Lingguibafa moxibustion is suprior to Mingmen moxibustion.