鸡胚尿囊膜黑色素移植瘤模型中微循环模式药物干预观察

Drugs Interference in Microcirculatory Patterns of Transplant Melanoma in Embryonic Chicken Model

目的:观察内皮抑素和多西环素对鸡胚尿囊膜黑色素移植瘤模型中不同微循环模式的影响,为黑色素瘤临床治疗提供实验依据。方法:分别于瘤细胞接种后3d(A组)和5d(B组)给予内皮抑素干预,于瘤细胞接种后3d(C组)和5d(D组)给予多西环素干预,给予无菌PBS溶液干预为对照组(E组),用HE和免疫组织化学染色的方法观察移植瘤中不同微循环模式面积的变化。结果:与E组比较,A组和B组移植瘤内内皮依赖性血管面积减小(P<0.01),A组血管生成拟态的面积增加(P<0.05);D组移植瘤内内皮依赖性血管和血管生成拟态的面积减少(P<0.05)。结论:内皮抑素能抑制内皮依赖性血管的生成,但不能抑制血管生成拟态的形成,而多西环素能同时抑制内皮依赖性血管和血管生成拟态的形成。

Objective： To study the effects of endostatin and doxycycline on microcirculatory patterns in the melanoma transplant model of the chorioallantoic membrane of the chicken embryo and the experimental evidences for melanoma therapy thereof. Methods： Endostatin was dripped on the chorioallantoic membrane after melanoma was transplanted 3 days （A group） and 5 days （B group）. Doxycycline was dripped on the chorioallantoic membrane after melanoma was transplanted 3 days （C group） and 5 days （D group）. The PBS solution was dripped on the chorioallantoic membrane in control group （E group）. The hematoxylin-eosin （HE）staining and immunohistochemical staining were performed to observe microcirculation patterns in sections. Results： Compared with the E group, the area of endothelium-dependent vessels were significantly decreased in A group and B group （P 〈 0.01）. But the areas of vasculogenic mimicry were bigger in the two groups（P 〈 0.05）. Compared with E group, the areas of endothelium-dependent vessels and vasculogenic mimicry were significantly decreased in D group （P 〈 0.05）. Conclusion： Endostatin can inhibit angiogenesis. But it has no effect on vasculogenic mimicry in the transplant melanoma. And Doxycycline can inhibit vasculogenic mimicry and angiogenesis in the transplant melanoma. The results provided experimental evidences for melanoma therapy.