2种方法制备小肽免疫原的免疫特性比较

Comparison of Immune Responses Elicited by Recombinant Fusion Hapten and Coupled Hapten

目的:探讨用不同方式制备的免疫原所产生的针对36肽半抗原的免疫应答特性。方法:分别用载体蛋白偶联小肽半抗原的方法与原核表达重组融合蛋白的方法构建免疫原免疫家兔,对其刺激产生的抗体应答动力学进行比较。结果:2种方法制备的免疫原免疫家兔后均于初次免疫后3个月左右产生了峰值效价,偶联蛋白方法制备的免疫原免疫家兔后产生的最高效价可达1∶12000,基因工程方法制备的免疫原免疫后最高效价为1∶6000,但后者诱导产生的免疫反应较为持久。结论:偶联蛋白方法与基因工程方法制备的免疫原均可成功刺激家兔免疫系统产生针对36肽半抗原的有效抗体,但两者具有较为不同的免疫特性,偶联蛋白方法更加迅速有效。

Objective ：To identify the immune characteristics elicited by immunogen prepared in two different ways against a 36AA-peptide. Methods：Rabbits were immunized by a 36-AA-hapten coupled with carrier proteins or recombinant fusion protein separately. The kinetics and specificities of antibody responses were compared. Results：After about 3 months of primary immunity, the antisera elicited by two kinds of immunogen reached the peak titer. The highest titer elicited by the immunogen prepared with coupled-hapten method reached 1：12 000, while the highest titer elicited by the immunogen prepared with gene engineering method was 1：6 000. But the immune response produced by the recombinant fusion protein was more long-lasting. Conclusion：Both kinds of immunogen can successfully elicit 36-AA specific antibody response in rabbits, which have different immune characteristics. To couple the hapten with vector proteins is a quicker and more effective way to prepare the immunogen.