人肝癌耐药细胞系的建立及生物学特性的研究

Construction and Characteristic of Human Multidrug-Resistant Hepatocellular Carcinoma Cell Line

目的:培养建立耐药肝癌细胞模型QGY7703/DDP,分析人肝癌耐药细胞系生物学特性的改变。方法:应用人肝癌细胞株QGY7703,采用顺铂(DDP)逐步提高加间歇诱导历时6个月在体外建立QGY7703/DDP细胞系模型。观察该细胞的生长规律,利用四甲基偶氮唑蓝(MTT)法分析暴露前后细胞的增殖和药物对细胞毒性的差异。结果:经过将QGY7703持续暴露,细胞对化疗药物5-氟尿嘧啶(5-FU)、DDP、阿霉素(ADM)、丝裂霉素(MMC)的敏感性发生了改变。QGY7703/DDP对5-FU的耐药性是亲代细胞的1.533倍,对DDP的耐药性是亲代细胞的2.181倍,对ADM的耐药性是亲代细胞的7.080倍,对MMC的耐药性是亲代细胞的9.461倍。结论:化疗药物能够诱导培养的肿瘤细胞产生耐药性。

Objective： To establish human multidrug-resistant hepatocellular carcinoma cell line （QGY7703/DDP） to analyze its different resistance and sensitivity. Methods： The human hepatocellular carcinoma cell line QGY7703 was continuously grown in the presence of cisplatin （DDP） for up to 6 months. Cell proliferation and cytotoxicity were serially monitored. Results： The chemosensitivities to 5-FU,DDP,adramycin （ADM） and mitomycin （MMC） were different between QGY7703 and QGY7703/DDP. The exhibitions were 1.533 fold to 5-FU, 2.181 fold to DDP, 7.08 fold to ADM, 9.461 fold to MMC in QGY？703/DDP. Conclusion： The drug resistance in cancer cell lines can be established by exposing to antitumor drugs in cell culture system.